Reaction kinetics of cephalexin synthesizing enzyme fromXanthomonas citri
- 1 July 1985
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 27 (7) , 953-960
- https://doi.org/10.1002/bit.260270705
Abstract
In enzymatic synthesis of cephalexin from D-α-phenylglycine methyl ester (PGM) and 7-amino-3-deacetoxy-cephalosporanic acid (7-ADCA) using α-acylamino-β-lactam acylhydrolase from Xanthomonas citri, it was found that this enzyme catalyzes all three reactions including PGM hydrolysis, cephalexin synthesis, and cephalexin hydrolysis. Based on our experimental results, a mechanistic kinetic model for cephalexin synthesizing enzyme system having acyl-enzyme intermediate was proposed. From this kinetic model, the reaction rate equations for three reactions were derived, and the kinetic parameters were evaluated. A good agreement between the simulation results and the experimental results was found.This publication has 31 references indexed in Scilit:
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