In vivo 31P‐NMR SPECTROSCOPY OF MAMMARY CARCINOMA SUBJECTED TO SUB CURATIVE PHOTODYNAMIC THERAPY

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Abstract
In vivo 31P‐NMR spectroscopy was applied to the investigation of the time course of metabolic response of MCA mammary carcinoma in the C,H mouse subjected to a subcurative treatment with photodynamic therapy. Eleven animals were injected with 12.5 mg kg_1 Photofrin II and after 24 h were photoactivated with 25 J cm−2 of light between 630 ± 5 nm. In vivo NMR spectroscopy was performed 1‐4, 24 and 72 h after treatment. The ratio of (J‐ATP intensity to inorganic phosphate (Pi) intensity changed over the time course of the study. At the 1‐4 and 24 h time points the ratio of fi‐ATP to Pi decreased from control values. At 72 h there was a significant increase in the ratio above the post treatment values to levels equal to or exceeding that of control pretreatment ratios.