RAPID AND SIMPLIFIED TECHNIQUE FOR THE ASSAY OF CHICKEN HEMOLYTIC COMPLEMENT

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 40  (3) , 443-445
Abstract
The technique described is a modification of a qualitative hemolytic radial diffusion technique. The test involves the use of sensitized sheep erythrocytes that were incorporated into agarose. Tube dilutions were made of chicken serum and samples of each dilution were placed into wells cut in the agarose. The test is quantitative for hemolytic complement in that the highest dilution showing visible hemolysis of sensitized erythrocytes in agarose is the endpoint for that serum sample. The test as compared with the standard tube assay was less sensitive by approximately 1 dilution. The advantages of speed, simplicity and cost more than offset the decrease in sensitivity of the test.