INVITRO ANALYSIS OF RESPONSE OF MULTICELLULAR TUMOR SPHEROIDS EXPOSED TO CHEMOTHERAPEUTIC-AGENTS INVITRO OR INVIVO

  • 1 January 1978
    • journal article
    • research article
    • Vol. 38  (11) , 3595-3598
Abstract
Multicellular tumor spheroids (MTS) [mouse mammary carcinoma MaC-11 and lung carcinoma Line 1] were exposed to chemotherapeutic agents in vitro (nitrogen mustard) or in vivo (cyclophosphamide) and analyzed in vitro in terms of altered growth patterns. Whether the MTS were exposed in vitro or in vivo, the major effect of the drugs was to induce a dose-dependent lag period before the normal MTS growth rate resumed. Exposure of MTS in the [mouse] peritoneal cavity to i.v. cyclophosphamide results in patterns similar to the in vitro exposure system, except that a host anti-MTS reaction was detected. In combination, these 2 methods allow the study of the responses of these complex tumor forms to chemotherapy.