Abstract
In the DNA-dependent RNA polymerase reaction, the RNA chains formed contain ribonucleoside triphosphates at their starting points and grow by the subsequent addition of ribonucleotides to the 3''-hydroxyl group of the ribonucleoside end. Purine nucleoside triphosphates are preferentially found at the triphosphate end. When denatured DNA is used as a template, there are an increase in the number and a change in the kind of starting points.