Effect of Brightener on Spore Germination

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Abstract
Substantial increases in the percent spore germination, as evidenced by colony count, followed a 2-hour exposure to brightener in 10 spp. of 5 genera of microorganisms. The average increase ranged from 159 to 848%, and the optimum concentration of brightener varied for the specific organism from 1.15 mg/ml to 10.9 mg/ml in contrast to a fluorescent labelling concentration of 0.05 mg/ml. The organisms in which this effect was obtained are: Aspergillus flavus ATCC 9170, A. fumgiatus ATCC 10894, Mucor murorum QM 776, Neurospora crassa ATCC 9683, Penicillium chrysogenum Wisc. Q 176, P. griseofulvum NRRL 2300, Streptomyces aureofaciens ATCC 10762, S. griseus ATCC 10137, S. lavendulae ATCC 8664, and Streptomyces sp. Lederle 6686-2.

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