ISOLIERUNG OESTROGENER STEROIDE AUS KÖRPERFLÜSSIGKEITEN LANDWIRTSCHAFTLICHER NUTZTIERE DURCH MIKROSUBLIMATION

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Abstract
From chromogenic compounds highly concentrated after separation, by repeated column-chromato-graphic rectification with Al2O3 and fractionation of the resulting extracts, as well as by reiterate transsublimation of the crystalline residues, crystals whose shapes were typical of different estrogenic fractions (estrone, 17 [alpha]-estradiol and 17 [beta]-estradiol) were obtained. They were identified by microscopical determination of their melting points. Estrone and 17 [beta]-estradiol from the urine of pregnant mares, estrone from the urine of pregnant cows and sows, estrone and 17[alpha]-estradiol from the amniotic liquor of the cow, estrone from the am-niotic liquor of swine, and estrone from the urine and the bile of pig-foetuses, were successfully isolated.

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