HISTOCHEMICAL STUDIES ON EFFECT OF THYROID-HORMONE ON ALKALINE AND ACID-PHOSPHATASE ACTIVITIES IN LIVER OF FISH AND AMPHIBIA

  • 1 January 1976
    • journal article
    • research article
    • Vol. 68  (1) , 80-85
Abstract
The lata fishes (Ophicephalus punctatus) showed increased alkaline and acid phosphatase activities in liver after immersion for 15-30 days in thyroxine-containing medium (0.025 .mu.g/ml). A single injection of thyroxine (1-2 .mu.g/g of body wt) caused increased acid phosphatase activity in liver of lata fish in comparison to the controls on the 5th day after experiment, but the alkaline phosphatase activity remained unchanged. Alkaline and acid phosphatases showed increased activites in liver of lata fishes treated with a single injection of 4 .mu.g of thyroxine/g of body wt on the 5th day. Immersion of lata fishes in thiourea solution (1 mg/ml) for 15 days did not show any alteration in alkaline or acid phosphatase activities, but these enzyme activities decreased after 30 days'' immersion in thiourea solution in comparison to the controls. A seasonal variation of alkaline and acid phosphatase activities was observed in liver of lata fishes. More alkaline phophatase activity was found in liver of summer fishes than in winter fishes. The winter fishes showed more acid phosphatase activity than the summer fishes. Three consecutive injections of thyroxine (0.1 .mu.g/g of body wt) to toads (Bufo melanostictus) caused increased alkaline and acid phosphatase activities in liver on the 5th day of the experiment, in comparison with the controls.

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