ACTIVATION OF RABBIT LIVER FRUCTOSE DIPHOSPHATASE BY COENZYME A AND ACYL CARRIER PROTEIN
- 1 November 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 64 (3) , 947-951
- https://doi.org/10.1073/pnas.64.3.947
Abstract
The catalytic activity of rabbit liver fructose diphosphatase is enhanced more than fourfold by treatment with coenzyme A or acyl carrier protein. Other sulfhydryl compounds, such as glutathione or cysteine, are without effect. Activation is reversed by reduced glutathione or cysteine, indicating that the enzyme and activator are linked by disulfide bridges. The activated enzyme derivative can be formed by a disulfide exchange reaction at alkaline pH or by an oxidation reaction which proceeds at neutral pH. The latter process requires O(2) and Cu(++), and is inhibited by EDTA.Keywords
This publication has 11 references indexed in Scilit:
- Studies on the subunit structure of rabbit liver fructose diphosphataseArchives of Biochemistry and Biophysics, 1969
- Fructose Diphosphatase from rabbit liver. IX. Isolation and kinetic properties of the enzyme-substrate complexBiochemistry, 1968
- Incorporation of pantothenate-1-14C into pigeon liver fatty acid synthetase: 4′-phosphopantetheine, a prosthetic group of the multienzyme complexArchives of Biochemistry and Biophysics, 1968
- Regulation of fructose diphosphatase activity by disulfide exchange.Proceedings of the National Academy of Sciences, 1967
- Structure and function in fructose diphosphatase.1966
- Fructose diphosphatase from rabbit liverArchives of Biochemistry and Biophysics, 1965
- Acyl carrier protein. V. Identification of 4'-phosphopantetheine bound to a mammalian fatty acid synthetase preparation.Proceedings of the National Academy of Sciences, 1965
- Allosteric Inhibition of Rat Liver Fructose 1,6-Diphosphatase by Adenosine 5'-MonophosphateJournal of Biological Chemistry, 1965
- FRUCTOSE DIPHOSPHATASE FROM RABBIT LIVER .I. PURIFICATION AND PROPERTIES1965
- FRUCTOSE DIPHOSPHATASE FROM RABBIT LIVER .2. CHANGES IN CATALYTIC PROPERTIES INDUCED BY DINITROFLUOROBENZENE1965