Purification of alkaline phosphatase by tryptic digestion

1 January 1949

journal article
research article
Published by Portland Press Ltd. in Biochemical Journal

Vol. 44 (4) , 434-435
https://doi.org/10.1042/bj0440434

Abstract

Further purification of the alkaline phosphatase, beyond that attainable by fractional precipitation and salting-out procedures, was obtained by incubation with 0.1-0.2% trypsin at 37[degree]C for a period of not more than 4 hrs. Addition of more trypsin, or incubation for longer periods, resulted in loss of enzyme activity.

Keywords

TRYPSIN

This publication has 2 references indexed in Scilit:

Purification of alkaline phosphatase
Biochemical Journal, 1949
STUDIES ON ACTIVATION OF PURIFIED ALKALINE PHOSPHATASE
1948