Mass spectrometric identification of C20 fatty acids bovine lens using the pyrrolidide derivative

Abstract
Unsaturated C20 fatty acids from bovine lens have been examined via gas chromatography‐mass spectrometry of their pyrrolidine derivatives. The presence of arachidonic acid and 5,8,11‐20∶3 in bovine lens has been confirmed by this study. In addition, the 10‐, 11‐, 12‐, and 13‐isomers of 20∶1 and the 7,13‐, 8,14‐, and 12,15‐isomers of 20∶2 were identified.