Both purified human 1,N6-ethenoadenine-binding protein and purified human 3-methyladenine-DNA glycosylase act on 1,N6-ethenoadenine and 3-methyladenine.
- 15 October 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (20) , 9386-9390
- https://doi.org/10.1073/pnas.89.20.9386
Abstract
We previously described a protein, isolated from human tissues and cells, that bound to a defined double-stranded oligonucleotide containing a single site-specifically placed 1,N6-ethenoadenine. It was further demonstrated that this protein was a glycosylase and released 1,N6-ethenoadenine. We now find that this enzyme also releases 3-methyladenine from methylated DNA and that 3-methyladenine-DNA glycosylase behaves in the same manner, binding to the ethenoadenine-containing oligonucleotide and cleaving both ethenoadenine and 3-methyladenine from DNA containing these adducts. The rate and extent of glycosylase activities toward the two adducts are similar.Keywords
This publication has 16 references indexed in Scilit:
- Partial purification of a human DNA glycosylase acting on the cyclic carcinogen adduct 1,N6-ethenodeoxyadenosine.1992
- Human cells contain protein specifically binding to a single 1,N6-ethenoadenine in a DNA fragment.Proceedings of the National Academy of Sciences, 1991
- Enzymic oxidation of ethyl carbamate to vinyl carbamate and its role as an intermediate in the formation of 1,N6-ethenoadenosineChemical Research in Toxicology, 1991
- Human cDNA expressing a functional DNA glycosylase excising 3-methyladenine and 7-methylguanineBiochemical and Biophysical Research Communications, 1991
- Role of human cytochrome P-450 IIE1 in the oxidation of many low molecular weight cancer suspectsChemical Research in Toxicology, 1991
- Further purification and characterization of human 3-methyladenine-DNA glycosylase Evidence for broad specificityBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1984
- Partial purification and characterization of 3-methyladenine-DNA glycosylase from human placentaBiochemistry, 1982
- DNA Repair EnzymesAnnual Review of Biochemistry, 1982
- Labeled 1,N6-ethenoadenosine and 3,N4-ethenocytidine in hepatic RNA of mice given [ethyl-1,2-3H or ethyl-1-14C] ethyl carbamate (urethan)Carcinogenesis: Integrative Cancer Research, 1982
- Human lymphoblasts contain DNA glycosylase activity excising N-3 and N-7 methyl and ethyl purines but not O6-alkylguanines or 1-alkyladenines.Proceedings of the National Academy of Sciences, 1981