Isolation of human serum low‐density lipoproteins with the aid of an immune‐specific adsorber
- 1 June 1970
- Vol. 5 (6) , 501-504
- https://doi.org/10.1007/bf02532735
Abstract
Human serum lipoproteins containing B‐protein have been isolated using an immunoadsorber. Bromoacetyl cellulose was combined with pure antibodies to low density lipoprotein (LDL) and an immunoadsorber of high capacity was obtained. With 1 g of this immunoadsorber all LDL and very low density lipoprotein (VLDL) from 30 ml pooled human serum were adsorbed and then eluted with glycine‐HCl buffer pH 3.2 at 0 C. The isolated lipoproteins were investigated by electrophoresis, immunodiffusion and ultracentrifugation, and found to be identical to LDL+VLDL isolated by ultracentrifugation.Keywords
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