Luciferin derivatives in bioluminescence‐enhanced enzyme immunoassays
- 1 July 1989
- journal article
- luminescence immunoassay
- Published by Wiley in Journal of Bioluminescence and Chemiluminescence
- Vol. 4 (1) , 119-128
- https://doi.org/10.1002/bio.1170040118
Abstract
Ultrasensitive bioluminescence immunoassays for the determination of peptides and proteins (illustrated with human urinary kallikrein, bradykinin and the determination of human urinary kallikrein antibody titres) have been developed. The usable ranges of the standard curves are from 5 pg to 5000 pg per litre. The relative intra-assay coefficients of variation of the tests were between 2% and 6%, and the inter-assay coefficients of variation between 4% and 12%.Keywords
This publication has 19 references indexed in Scilit:
- Immunoassays für die Spurenanalyse niedermolekularer VerbindungenAnalytical and Bioanalytical Chemistry, 1984
- Use of enzymes in immunoassay techniques. A reviewThe Analyst, 1984
- Biological diversity, chemical mechanisms, and the evolutionary origins of bioluminescent systemsJournal of Molecular Evolution, 1983
- The limitations of radioimmunoassay (RIA)TrAC Trends in Analytical Chemistry, 1982
- Recent advances in homogeneous and separation-free enzyme immunoassaysApplied Biochemistry and Biotechnology, 1981
- Radioimmunoassay: A Probe for the Fine Structure of Biologic SystemsScience, 1978
- Continuous monitoring of ATP-converting reactions by purified firefly luciferaseAnalytical Biochemistry, 1976
- Zum Problem der NachweisgrenzeAnalytical and Bioanalytical Chemistry, 1965
- The Structure and Synthesis of Firefly LuciferinJournal of the American Chemical Society, 1963
- Assay of Plasma Insulin in Human Subjects by Immunological MethodsNature, 1959