Multisite serine phosphorylation of the insulin and IGF‐I receptors in transfected cells
- 19 August 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 288 (1-2) , 206-211
- https://doi.org/10.1016/0014-5793(91)81035-7
Abstract
Serine phosphorylation of insulin/IGF‐I receptors in transfected fibroblasts was analysed by peptide mapping, PMA stimulated the phosphorylation of 5 distinct insulin receptor phosphopeptides: a single major phosphothreonine peptide containing Thr‐1348, one major and 3 minor phosphoserine peptides. The major insulin‐stimulated phosphoserine peptides were the same as those after PMA, with the exception of 2 minor phosphoserine peptides. PMA stimulated phosphorylation of a single major IGF‐I receptor phosphoserine peptide which was phosphorylated to a lesser extent after IGF‐I. We conclude that insulin/IGF‐I and PMA stimulate phosphorylation of the same sites, but differ in the extents of phosphorylation.Keywords
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