Reactive blue 2 selectively inhibits P2y‐purinoceptor‐stimulated surfactant phospholipid secretion from rat isolated alveolar Type II cells
Open Access
- 30 April 1989
- journal article
- research article
- Published by Wiley in British Journal of Pharmacology
- Vol. 97 (1) , 158-162
- https://doi.org/10.1111/j.1476-5381.1989.tb11937.x
Abstract
1 The effect of reactive blue 2 on adenosine 5′-triphosphate (ATP), 12-O-tetradecanoylphorbol 13-acetate (TPA) and terbutaline-induced surfactant phospholipid secretion from rat isolated alveolar Type II cells was studied. 2 Reactive blue 2 significantly inhibited ATP-induced surfactant phospholipid secretion, but was without effect on C-kinase agonist (TPA) or β-adrenoceptor agonist (terbutaline)-stimulated surfactant phospholipid secretion. The IC50 for inhibition of ATP-induced surfactant secretion was 1.5 × 10−4 m. 3 These data are consistent with a P2y-purinoceptor regulating surfactant phospholipid secretion from isolated Type II cells and support previous work suggesting reactive blue 2 is a specific inhibitor at P2y-purinoceptors.This publication has 18 references indexed in Scilit:
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