Superoxide dismutases from kidney bean leaves
- 1 November 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 20 (7) , 1229-1235
- https://doi.org/10.1093/oxfordjournals.pcp.a075922
Abstract
Three isozymes of superoxide dismutase were found in the soluble extract of kidney bean leaves. Two of them, purified to near homogeneous states, were inhibited by cyanide and by the antibody to spinach Cu, Zn-superoxide dismutase. Thus, both isozymes were the Cu and Zn containing enzyme that has a molecular weight of 32,000 and is composed of two subunits of equal size. Another isozyme was insensitive to cyanide and to the antibody, but was inactivated by acetone or heating. The cyanideinsensitive isozyme was not inactivated by H2O2 showing that this isozyme is Mn-superoxide dismutase.Keywords
This publication has 2 references indexed in Scilit:
- Superoxide dismutases in photosynthetic organisms: Absence of the cuprozinc enzyme in eukaryotic algaeArchives of Biochemistry and Biophysics, 1977
- Superoxide dismutase: Improved assays and an assay applicable to acrylamide gelsAnalytical Biochemistry, 1971