A simple reproducible method for prometaphase chromosome analysis
- 1 January 1982
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 60 (4) , 328-333
- https://doi.org/10.1007/bf00569213
Abstract
A method is described for the analysis of chromosomes in prophase and early metaphase. It involves culturing the lymphocytes in medium RPMI-1640, supplemented with 10% autologous plasma instead of fetal bovine serum. Living cells are treated with actinomycin D and colcemid for 1 h prior to harvest and harvested early at 65 h of incubation, using a hypotonic solution formulated by Ohnuki (1968). The method has been tested on several hundred clinical samples on a routine basis. On average, 30% of the dividing cells were in prometaphase.This publication has 39 references indexed in Scilit:
- High-resolution bands in human fibroblast chromosomes induced by actinomycin DCytogenetic and Genome Research, 1981
- Pachytene chromomere maps of human chromosomes 19 and 20Cytogenetic and Genome Research, 1980
- Chromosome structure and function in manCytogenetic and Genome Research, 1978
- Preferred base sites of actinomycin-deoxyribonucleic acid bindings by circular dichroismJournal of the American Chemical Society, 1976
- Mapping G-bands on human prophase chromosomesCytogenetic and Genome Research, 1975
- Chromosome structure and function in manCytogenetic and Genome Research, 1971
- The action of proflavin and actinomycin D in causing chromatid breakage in human cellsExperimental Cell Research, 1965
- Experimental alteration of metaphase chromosome morphologyExperimental Cell Research, 1965
- Human chromosome uncoiling and dissociationAnnals of Human Genetics, 1962
- CHROMOSOME ANALYSIS FROM SMALL VOLUMES OF BLOODThe Lancet, 1961