Stereochemistry of the Reduction Step Mediated by Recombinant 1-Deoxy-d-xylulose 5-Phosphate Isomeroreductase
- 31 August 1999
- journal article
- letter
- Published by American Chemical Society (ACS) in Organic Letters
- Vol. 1 (6) , 921-923
- https://doi.org/10.1021/ol990839n
Abstract
The stereochemistry of the 1-deoxy-d-xylulose 5-phosphate (DXP) isomeroreductase reduction step has been examined using the recombinant enzyme from Synechocystis sp. PCC6803. Using [3-2H]DXP and [4S-2H]NADPH, it has been determined that the C1 pro-S hydrogen in the 2-C-methyl-d-erythritol 4-phosphate product derives from C3 of DXP, indicating that hydride attack occurs on the re face of the intermediate aldehyde. The 4S-hydride from NADPH is delivered, assigning this enzyme as a class B dehydrogenase.Keywords
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