Biochemical basis of heart function. XII. Sodium-potassium stimulated adenosine triphosphatase activity in the perfused rat heart made to fail by substrate-lack

Abstract
The activity of Na+−K+ stimulated ATPase obtained from the failing rat heart due to substrate-lack was lower than that in heart perfused with medium containing glucose. Depressed contractile force and Na+−K+ ATPase activity of the hearts perfused with substrate-free medium for 1 hr were reversible on further perfusion of the hearts with medium containing glucose. The results indicate the role of exogenous substrate in the maintenance of Na+−K+ ATPase activity in the myocardium and lend further support to our contention concerning membrane abnormality in heart failure.

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