Isolation of the Fourth Component (C4) of Rat Complement
Open Access
- 1 November 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 123 (5) , 2261-2264
- https://doi.org/10.4049/jimmunol.123.5.2261
Abstract
The fourth component of rat complement was purified to homogeneity by sequential chromatography of rat plasma in benzamidine on QAE-A50, SP-C50, hydroxyapatite, and gel filtration on Bio-Gel A 1.5. The final material was homogeneous on SDS-PAGE analysis and had a calculated m.w. of 198,000. A monospecific antibody against rat C4 was obtained from immunized rabbits. The concentration of rat C4 in the plasma of normal 4-month-old Wistar rats was 190 ± 34 µg/ml (mean ± 1 S.D.).This publication has 1 reference indexed in Scilit: