Recombinant murine steel factor stimulates in vitro production of granulocyte–macrophage progenitor cells

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Abstract
The ability of murine Steel factor to promote the in vitro production of granulocyte‐macrophage progenitor cells (CFU‐GM) was examined in short‐term liquid cultures. Bone marrow from C57BL/6J or Sl/Sld mice was placed in culture for seven days with either Steel factor alone or in the presence of IL‐3. CFU‐GM responsive to GM‐CSF, IL‐3, and CSF‐1 were measured in the input population and again after 3 or 7 days in culture. Steel factor alone increased the number of all CUF‐GM types as early as 3 days after culture initiation, with further increases at day 7. This effect was protentiated by the addition of IL‐3. Production of CFU‐GM by C57BL/6J or Sl/Sld marrow was comparable except for enhanced production of CSF‐1 responsive progenitors by Sl/Sld marrow. A recombinant Sld protein was also shown to be equivalent to the wild‐type protein in its capacity to promote CFU‐GM production from normal bone marrow.