Red Fluorescent Protein (DsRed) as a Reporter in Saccharomyces cerevisiae
Open Access
- 15 June 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (12) , 3791-3794
- https://doi.org/10.1128/jb.183.12.3791-3794.2001
Abstract
We describe the utilization of a red fluorescent protein (DsRed) as an in vivo marker for Saccharomyces cerevisiae. Clones expressing red and/or green fluorescent proteins with both cytoplasmic and nuclear localization were obtained. A series of vectors are now available which can be used to create amino-terminal (N-terminal) and carboxyl-terminal (C-terminal) fusions with the DsRed protein.Keywords
This publication has 23 references indexed in Scilit:
- High efficiency transformation of Escherichia coli with plasmidsPublished by Elsevier ,2003
- Construction of a genomic library of the food spoilage yeastZygosaccharomyces bailiiand isolation of the β-isopropylmalate dehydrogenase gene (ZbLEU2)FEMS Yeast Research, 2001
- THE GREEN FLUORESCENT PROTEINAnnual Review of Biochemistry, 1998
- Chapter 9: GFP Variants for Multispectral Imaging of Living CellsPublished by Elsevier ,1998
- Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicansMicrobiology, 1997
- Comparative mutagenesis of nuclear localization signals reveals the importance of neutral and acidic amino acidsCurrent Biology, 1996
- Green‐fluorescent protein mutants with altered fluorescence excitation spectraFEBS Letters, 1995
- Green Fluorescent Protein as a Marker for Gene ExpressionScience, 1994
- [40] Immunofluorescence methods for yeastPublished by Elsevier ,1991
- Cheap and simple yeast mediaJournal of Microbiological Methods, 1986