Identification and Isolation of Melanoma-Associated Antigens with Monoclonal Antibodies

Abstract
The monoclonal antibodies distributed at the first Workshop on Monoclonal Antibodies to Melanoma have been tested by immunoprecipitation, and one group of antigens (90 kd) has been found to be identical by sequential immunoprecipitation. These results are in good agreement with those from other laboratories. In addition, antigens from a new group of hybridomas, generated since the first Workshop, were studied. Some of these antigens are unique and others are duplicates of the original set. Finally, a melanomaassociated antigen (260 kd) was purified by immunoaffinity chromatography.

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