A Simplified Calibration Procedure for Elution Chromatography on Gel Filtration Columns

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Abstract
For elution chromatography on gel filtration columns, a simple plot of migration rate vs. Stokes radius provides nearly as good a straight line fit as the more complicated plot of inverse error function complement of partition coefficient (K) vs. Stokes radius. For a matrix of known characteristics only two standards are needed, one large and one small, to provide a calibration independent of, and hence relatively insensitive to, void and included volumes of the particular column being calibrated. With real data, the simple method appears as reliable as the more complex one.