Novel method for substance injection into the cell by laser beam. A study of the injection volume.
- 31 December 1985
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 11 (2) , 205-207
- https://doi.org/10.1247/csf.11.205
Abstract
A DNA transfection method by laser microbeam pricking has been recently reported (Kurata, S. et al. Exp. Cell Res. 162, 372 (1986)). The volume of external fluid transferred into the cell by the method was determined through the injection of diphtheria toxin fragment A (Yamaizumi, M. et al. Cell 15, 245 (1978)). Using these results and the results on laser DNA transfection efficiency (Kurata, S. et al., Exp. Cell Res. 162, 372 (1986)), the approximate number of DNA molecules necessary to transform the recipient cell was estimated.This publication has 4 references indexed in Scilit:
- The laser method for efficient introduction of foreign DNA into cultured cellsExperimental Cell Research, 1986
- The ‘pricking’ methodExperimental Cell Research, 1982
- Intracellular introduction of a fixed quantity of substances by pricking cells using a modified microscopeExperimental Cell Research, 1981
- One molecule of diphtheria toxin fragment a introduced into a cell can kill the cellCell, 1978