Fundamental Research for the Pharmacological Activity of Oriental Drugs. XI. Combined Effects of Animal-Origin Drugs on the Inhibition of Leucocyte Migration and Their Effective Components

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Abstract
Combined effects of drugs of animal origin (musk, bezoar and bufotalis) were examined by Boyden''s method, using guinea pig polymorphonuclear leukocytes for inhibitory effects on leukocyte migration. A migration-inhibitory activity was found in musk, bezoar, ninhydrin reaction-positive substances of musk peptide fraction (MP) extracted from musk and smooth muscle contracting substances extracted from bezoar. MP was the most effective substance tested. The active component of MP was determined to be a peptide from the fact that the activity was lost by tryptic hydrolysis and that the MW was estimated to be about 1000, using ultrafiltration and Sephadex. The combined action of musk-bezoar was an antagonistic type. No combined action of musk-bufotalis was observed, but bezoar-bufotalis was synergistic. The apparent combined action of musk-bezoar-bufotalis was not observed, but the interactions described above seem at least to be included.