Human Monoclonal Immunoglobulin M Antibodies to Ganglioside GM1Show Diverse Cross-Reactivities with Lipopolysaccharides ofCampylobacter jejuniStrains Associated with Guillain-Barré Syndrome

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Abstract
We examined the reactivity of a panel of anti-GM1immunoglobulin M monoclonal antibodies (MAbs) cloned from multifocal motor neuropathy patients with lipopolysaccharides (LPSs) ofCampylobacter jejuni strains, including serotype O:41 strains associated with Guillain-Barré syndrome. The MAbs reacted with ganglioside GM1 to different degrees, and these differences in fine specificities for GM1 were reflected in the different degrees of reactivity with each of the C. jejuni LPSs tested. Antibodies could also be discriminated by the varying patterns of inhibition by cholera toxin (a GM1ligand) in LPS binding studies. These results indicate that there is a substantial heterogeneity among C. jejuni O:41 strains in their expression of GM1-like epitopes and among the fine specificities of different neuropathy-associated anti-GM1antibodies.