Abstract
Comparative cytological studies of several spp. of aerobic and anaerobic spore-forming bacteria showed significant differences. The cell of the aerobic spp. is thicker than that of the anaerobic ones, and forms lipoprotein granules which contain reserve substances. These granules are formed independently of the contents of the medium, but increase in size in substrates rich in carbohydrates. They are soluble in acetic acid or acetone, and insoluble in alcohol, ether or chloroform. The cell of anaerobic spp. appears homogeneous. Its chromatin is diffuse, but precipitates out and forms a small corpuscle (prospore) which gradually becomes the spore. It never forms lipoprotein granules. Carbohydrate reserve material occurs chiefly in the cell of the anaerobic spp. and is used up during spore formation.

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