Microsome-dependent methylation of erythrocyte proteins by dimethylnitrosamine
- 1 January 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 2 (3) , 179-182
- https://doi.org/10.1093/carcin/2.3.179
Abstract
The precarcinogen, N-[methyl- 14 C]dimethylnitro-samine, rat liver microsomes and NADPH were incubated in the presence of intact human erythrocytes. Uptake of radioactivity into erythrocytes was time-dependent and analysis of an acid hydrolysate of the erythrocyte proteins showed several radioactive peaks which were identified as 3-N-methyl- and 1-N-methyl-histidine, and S-methyl-cysteine. Since no binding occurs in the absence of microsomes, the alkylating species from dimethylnitrosamine metabolism can clearly penetrate the erythrocyte plasma membrane.Keywords
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