Marrow Cells from Patients with Untreated Pernicious Anaemia Cannot Use Tetrahydrofolate Normally

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Abstract
Folate analogs were added to human bone marrow cells to determine their effect on deoxyuridine [dU] utilization in the deoxyuridine suppression test. Formyltetrahydrofolates fully corrected the impairment of dU utilization in pernicious anemia marrows, but tetrahydrofolate was relatively ineffective. All these analogs were effective in megaloblastic marrows from folate deficient patients. Formyltetrahydrofolates enhanced dU utilization by normal human marrows, whereas methyltetrahydrofolate reduced its use. In terms of the methylfolate trap hypothesis, the expectation that cobalamin-deficient marrows would be able to use tetrahydrofolate normally was not realized.

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