Functional Culture of Human Periodontal Ligament Fibroblast (Hplf) on Polyelectrolyte Complex (Pec)

Publisher Website
Google Scholar
Abstract
Cell adhesion onto PEC was mainly controlled by component polyelectrolytes of PEC. In the combination of a synthetic polycation with strong basic groups (2X and PVBMA) and carboxymethyl chitin (CCHN), probably due to the cationic sites of PEC adhesion and growth of human periodontal ligament fibroblast (HPLF) were low and the cell morphology was a round form. Using chitosan (CS100) as a polycation, cell adhesion was scarcely decreased but cell growth was reduced, and cell aggregates were observed. Using sulfated chitin (SCHN) as a polyanion, in spite of a species of polycation, adhesion, growth and spreading form of HPLF were almost the same as those on tissue culture dish (TCD). Cell adhesion onto PEC containing CS100 was not affected by divalent cations. But the amount of adsorbed fibronectin onto such PECs depended upon a kind of polyanion; that onto sulfated chitin's PEC was 2–3 times as much as that onto carboxymethyl chitin's PEC. So it was suggested that cell adhesion was controlled by non specific interaction between PEC and cell, but cell growth was done by adsorbed fibronectin onto PEC. Moreover, cell morphology was seemed to be controlled by functional groups of polyanion.