TECHNETIUM-99M METHOXYISOBUTYL ISONITRILE (RP30) FOR QUANTIFICATION OF MYOCARDIAL ISCHEMIA AND REPERFUSION IN DOGS

  • 1 September 1988
    • journal article
    • research article
    • Vol. 29  (9) , 1539-1548
Abstract
This study was done to determine whether [99mTc]methoxyisobutyl isonitrile (RP30), a nonredistributing myocardial perfusion agent, could be used to quantify regional myocardial blood flow distribution during ischemia and reperfusion, employing sequential injections of tracer, tomographic imaging, and appropriate image subtraction. Dogs underwent transient (6 min) coronary artery occlusion, followed by two paired injections of RP30 and radioactive microspheres combined with tomographic imaging, the first during coronary occlusion and the second after 60 min reperfusion. To obtain a true image representative of reperfusion, the first set of images was corrected for 99mTc decay and substracted from the second. During occlusion, tissue microsphere content and scintigraphic RP30 activity in the center of the ischemic region (both expressed as a fraction of the nonischemiic region) were closely correlated, although RP30 consistently exceeded microsphere content (0.43 .+-. 0.03 vs. 0.24 .+-. 0.04, p < 0.01). Direct tissue counting of RP30 confirmed its relative excess in ischemic myocardium. Reperfusion was successful in 7/8 dogs, with an increase in RP30 activity to 0.98 .+-. 0.04 compared to 0.89 .+-. 0.03 for microspheres (p = N.S.). In one dog with microsphere-documented perisistent ischemia, the RP30 defect was still present after reflow. Our results indicate that because of the lack of myocardial clearance and redistriubtion, repeat injections of RP30 can be used to quantify serial changes in regional myocardial blood flow.