Cytokinin-Induced Ethylene Biosynthesis in Nonsenescing Cotton Leaves
Open Access
- 1 December 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 82 (4) , 930-935
- https://doi.org/10.1104/pp.82.4.930
Abstract
The influence of cytokinins on ethylene production was examined using cotton leaf tissues. Treatment of intact cotton (Gossypium hirsutum L. cv LG 102) seedlings with both natural and synthetic cytokinins resulted in an increase in ethylene production by excised leaves. The effectiveness of the cytokinins tested was as follows: thidiazuron ≫ BA ≫ isopentyladenine ≥ zeatin ≫ kinetin. Using 100 micromolar thidiazuron (TDZ), an initial increase in ethylene production was observed 7 to 8 hours post-treatment, reached a maximum by 24 hours and then declined. Inhibitors of 1-aminocyclopropane-1-carboxylic acid (ACC) synthesis and its oxidation to ethylene reduced ethylene production 24 hours post-treatment; however, by 48 hours only inhibitors of ACC oxidation were effective. The increase in ethylene production was accompanied by a massive accumulation of ACC and its acid-labile conjugate. TDZ treatment resulted in a significant increase in the capacity of tissues to oxidize ACC to ethylene. Endogenous levels of methionine remained constant following TDZ treatment. It was concluded that the stimulation of ethylene production in cotton leaves following cytokinin treatment was the result of an increase in both the formation and oxidation of ACC.This publication has 13 references indexed in Scilit:
- Quantitative Analysis of Pathways of Methionine Metabolism and Their Regulation in LemnaPlant Physiology, 1985
- Involvement of Ethylene in the Action of the Cotton Defoliant ThidiazuronPlant Physiology, 1985
- Effect of the Defoliant Thidiazuron on Ethylene Evolution from Mung Bean Hypocotyl SegmentsPlant Physiology, 1984
- Enhancement by Ethylene of Cellulysin-Induced Ethylene Production by Tobacco Leaf DiscsPlant Physiology, 1984
- The effect of plant-hormone pretreatments on ethylene production and synthesis of 1-aminocyclopropane-1-carboxylic acid in water-stressed wheat leavesPlanta, 1982
- Cytokinin activity of N-phenyl-N′-1, 2,3-thiadiazol-5-ylurea (thidiazuron)Phytochemistry, 1982
- Methionine metabolism and ethylene biosynthesis in senescing petals of TradescantiaPhytochemistry, 1980
- Auxin-induced ethylene biosynthesis in subapical stem sections of etiolated seedlings of Pisum sativum L.Planta, 1979
- The effect of 6-benzyladenine and leaf ageing treatment on the levels of stress-induced ethylene emanating from wilted wheat leavesPlanta, 1979
- Effects of Kinetin, IAA, and Gibberellin on Ethylene Production, and Their Interactions in Growth of SeedlingsPlant Physiology, 1968