Amino Acid Sequence of Immunity Protein (B Subunit) of ColicinE31
- 31 March 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 89 (5) , 1609-1618
- https://doi.org/10.1093/oxfordjournals.jbchem.a133356
Abstract
The amino acid sequence of immunity protein (protein B) of colicin E3 (84 amino acid residues) was determined. B was first split into two fragments, CB 1 and CB 2, by CNBr treatment. The structure of CB 1 (the N-terminal part of B) was determined by Edman degradation of whole protein B. The structure of CB 2 (the C-terminal part of B) was established by sequencing its four trypsin peptides. The sequences and the alignment of the tryptic peptides were completed by analyses of thermolysin peptides of CB 2. These results established the complete amino acid sequence of B.This publication has 4 references indexed in Scilit:
- Role of mammalian RNase inhibitor in cell-free protein synthesisProceedings of the National Academy of Sciences, 1979
- Amino Acid Sequence of an Active Fragment (T2A) of Colicin E31The Journal of Biochemistry, 1978
- Ribonuclease inhibitor from human placenta. Purification and properties.Journal of Biological Chemistry, 1977
- [8] End-group analysis using dansyl chloridePublished by Elsevier ,1972