Molecular cloning and expression inE. coliof aSalmonella typhiporin gene
- 29 February 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 229 (1) , 77-81
- https://doi.org/10.1016/0014-5793(88)80801-9
Abstract
Immunoscreening of a Salmonella typhi cosmid library in E. coli allowed the detection of clones producing a 36 kDa porin from S. typhi. The gene is efficiently expressed in an E. coli porin-less mutant and the protein is exported to the outer membrane envelope. Two clones which markedly differ in their level of expression have been isolatedKeywords
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