Degradation of [3H]thymidine by a pentosyltransferase (EC 2.4.2.4) in the plasma of man and different animals
- 1 May 1977
- journal article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 33 (5) , 668-670
- https://doi.org/10.1007/bf01946565
Abstract
[3H]Thymidine is degraded by an enzyme (thymidine phosphorylase; EC 2.4.2.4) which we have identified in the plasma of man and some animals. The presence of this enzyme in plasma or sera used to supplement culture media may, under certain experimental conditions, limit the validity of measuring the uptake of radiolabeled thymidine as a means of defining DNA synthesis.Keywords
This publication has 11 references indexed in Scilit:
- ELABORATION OF PYRIMIDINE-SPECIFIC NUCLEOSIDASES BY HUMAN LYMPHOBLASTOID CELLS OF ESTABLISHED CULTURES1976
- Purification of thymidine phosphorylase from Escherichia coli and its photoinactivation in the presence of thymine, thymidine, and some halogenated analogs.Journal of Biological Chemistry, 1975
- Pyrimidine nucleoside phosphorylase during development of the normal and dystrophic chickenBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Pentosyl Transfer Mechanisms of the Mammalian Nucleoside PhosphorylasesJournal of Biological Chemistry, 1968
- PYRIMIDINE NUCLEOSIDE PHOSPHORYLASES OF RAT LIVER - SEPARATION BY ION EXCHANGE CHROMATOGRAPHY AND STUDIES OF EFFECT OF CYTIDINE OR URIDINE ADMINISTRATION1968
- ENXYMATIC MECHANISMS FOR DEOXYTHYMIDINE SYNTHESIS IN HUMAN LEUKOCYTES .I. SUBSTRATE INHIBITION BY THYMINE AND ACTIVATION BY PHOSPHATE OR ARSENATE1967
- The Incorporation and Degradation of Pyrimidine DNA Precursors by Human LeucocytesBritish Journal of Cancer, 1964
- Thymidine Catabolism by Normal and Leukemic Human Leukocytes*Journal of Clinical Investigation, 1964
- DEOXYRIBOSYL TRANSFER .I. THYMIDINE PHOSPHORYLASE + NUCLEOSIDE DEOXYRIBOSYLTRANSFERASE IN NORMAL + MALIGNANT TISSUES1964
- THE ENZYMATIC SYNTHESIS OF NUCLEOSIDES .1. THYMIDINE PHOSPHORYLASE IN MAMMALIAN TISSUE1954