Intraoperative isolation and processing of BM-derived stem cells

Publisher Website
Google Scholar
Abstract
To improve tissue regeneration of ischemic myocardium, autologous bone marrow-derived stem cells have been injected intramyocardially in five patients undergoing coronary artery bypass grafting and transmyocardial laser revascularization. An innovative method for the intraoperative isolation ofCD133+ -stem cells in less than 3 hours has been established. After induction of general anesthesia, approx. 60–240ml of bone marrow were harvested from the posterior iliac crest and processed in the operating room under GMP conditions using the automated cell selection device Clini-MACS. Following standard CABG surgery, LASER channels were shot in predefined areas within the hibernating myocardium. Subsequently, autologous CD133+-stem cells (1.9–9.7×106 cells; purity up to 97%) were injected in a predefined pattern around the laser channels. Through the intraoperative isolation of CD133+ -cells, this effective treatment of ischemic myocardium can be applied to patients scheduled both for elective and for emergency revascularisation procedures.