RADIOIMMUNOASSAY OF HUMAN PANCREATIC AMYLASE IN SERUM AND URINE

  • 1 January 1980
    • journal article
    • research article
    • Vol. 29  (3) , 513-525
Abstract
The purification of human pancreatic amylase (EC 3.2.1.1) and the development of a radioimmunoassay for this enzyme is described. The enzyme was purified from a normal human pancreas obtained at autopsy and antibodies were produced in rabbits. The purified pancreatic amylase was radioiodinated and the radioimmunoassay was developed. Analytic recoveries in serum and urine specimens were 99 and 96%, respectively, and day-to-day precision < 10%. The assay gave a 20% crossreactivity with salivary amylase.

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