ISOMERIZATION OF VITAMIN A BY TISSUE HOMOGENATES

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Abstract
Homogenates of liver, intestinal mucosa, and kidney were shown to catalyze the conversion of 11-cis to all-trans vitamin A. The liver contained the most active system and converted approximately 42% of the 11-cis in a 3-hour incubation. Intestinal mucosa and kidney preparations promoted conversions of 20% and 9% respectively. Only liver homogenates had an appreciable effect on the 13-cis isomer and caused a 15% conversion to all-trans vitamin A. The activity of all preparations was destroyed by boiling and renewed by the addition of unboiled homogenate. The extent of isomerization was dependent on the concentration of both homogenate and vitamin A. These results are compared to those found in vivo.

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