DIRECT EVIDENCE FOR A CYTOPLASMIC MEMBRANE IN SECTIONED BACTERIA

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Abstract
In one of 3 embeddings of a species of Spirillum, isolated from river water, there was a clearly defined and strongly scattering membrane always closely applied to the surface of the cytology. This was distinct and separate from the cell wall, which was partially disintegrated as well as separated from the protoplast owing to a combination of shrinkage during dehydration, polymerization damage, and solution during treatments. It is postulated that it represents a true cytoplasmic membrane because of its location and the apparent separation from cell wall. Its thickness approximates 60 A.

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