Pig leukocyte cysteine proteinase inhibitor (PLCPI), a new member of the stefin family
- 27 December 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 336 (2) , 289-292
- https://doi.org/10.1016/0014-5793(93)80822-c
Abstract
A new stefin type low‐M r, cysteine proteinase inhibitor (PLCPI) was isolated from pig polymorphonuclear leukocytes as a contaminant of the cathelin sample. The inhibitor consists of 103 amino acids, and its M r, was calculated to be 11,768. The inhibitor exhibits considerable sequence identity with inhibitors from the stefin family, particularly with human stefin A. The PLCPI is a fast acting inhibitor of papain and cathepsins L and S (k ass ⩾ 1 × 106 M−1 · s−1) and forms very tight complexes with these enzymes (K i, ⩽ 190 pM). The affinity for cathepsins B and H (K i ⩾ 125 nM) was lower. These results also show that the inhibitory activity previously ascribed to cathelin was due to the presence of PLCPI.Keywords
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