Effects of radiation pasteurization on Salmonella. I. Parameters affecting survival and recovery from chicken

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Abstract
Growth of Salmonella typhimurium, strain RIA, in minimal broth supplemented with methionine, produced a population of cells that was more sensitive to 60Co irradiation than were those grown in brain heart infusion. The resistance values (D) were 0.056 and 0.066 Mrad, respectively. D was not significantly different when the bacteria were irradiated at 4 °C on fresh or frozen-thawed germ-free chicken, or on conventional chicken sterilized by autoclaving, or by exposure to 2 Mrad of ionizing radiation. It was generally higher when the cells were irradiated in the original growth medium than on chicken. D of five different strains of S. typhimurium irradiated at 4 °C on autoclaved chicken varied from 0.052 to 0.068 Mrad. The greatest increase in resistance was produced by decreasing the temperature during irradiation. S. typhimurium, RIA, attained a D value of 0.293 Mrad at −80 °C. The effect of the recovery media on survival is equivocal. In some instances nutrient and minimal agar seemed to provide slightly better recovery of strains SR-11 and RIA. Enrichment of complex media with yeast extract did not increase recovery. The results indicate that of those parameters studied, the most significant effects on resistance were due to the temperature during irradiation.

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