Routine Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry Assay for Simultaneous Measurement of the 25-Hydroxy Metabolites of Vitamins D2 and D3
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Open Access
- 1 September 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 51 (9) , 1683-1690
- https://doi.org/10.1373/clinchem.2005.052936
Abstract
Background: Measurement of 25-hydroxyvitamin D2 and D3 (25-OH D2 and D3) is essential for investigating vitamin D deficiency. Competitive binding techniques are unable to distinguish between the 2 metabolites and suffer from interference from other hydroxy metabolites of vitamin D.Keywords
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