Follicular Control of Meiosis in the Porcine Oocyte1

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Abstract
Organ culture methods using segments of follicle wall and oocytes were employed to study follicular meiosis control in porcine oocytes. Follicle wall segments maintained dictyate arrest in oocytes, an action which was specific to the follicle hemisections and not shown by control tissue. Luteinizing hormone [lutropin] induced meiotic resumption in this in vitro system. The cumulus oophorus was necessary to prevent oocyte maturation in vitro consistently when follicle wall was present.