Sodium/Potassium Adenosine Triphosphatase α-Subunit and α-Subunit mRNA Levels in Early Rabbit Embryos1
- 1 March 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 42 (3) , 539-544
- https://doi.org/10.1095/biolreprod42.3.539
Abstract
Sodium/potassium adenosine triphosphatase (Na+/K+ ATPase) and Na+/K+ ATPase mRNA content of rabbit embryos during preimplantation development were evaluated. Changes in Na+/K+ ATPase .alpha.-subunit content were detected with Western blotting using polyclonal antiserum against guinea pig Na+/K+ ATPase. Total RNA samples from rabbit embryos were analyzed by using Northern blots hybridized with random primer-labeled cDNA for Na+/K+ ATPase .alpha.-subunit from sheep kidney. Northern blots exhibited a single mRNA band (3.65 kilobases) in sheep kidneys and rabbit embryos. Between Day 4 and Day 6 of development, Na+/K+ ATPase .alpha.-subunit mRNA content increased 35-fold whereas Na+/K+ ATPase .alpha.-subunit content increased 22-fold. The similar increase in Na+/K+ ATPase .alpha.-subunit mRNA and .alpha.-subunit content in rabbit embryos suggests that Na+/K+ ATPase is partly regulated at the mRNA level during blastocyst expansion.Keywords
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