In vivo transfer of the Ti-plasmid of Agrobacterium tumefaciens to Escherichia coli
- 24 July 1978
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 163 (3) , 335-338
- https://doi.org/10.1007/bf00271963
Abstract
The Ti-plasmids are naturally self-transmissible from their normal host Agrobacterium to E. coli. They are however unable to stably establish themselves as a replicon in E. coli. It is nevertheless possible to study the Ti-plasmids in E. coli with the help of Ti::RP4 cointegrate plasmids that transfer and maintain themselves very efficiently in E. coli. An E. coli harbouring such a Ti::RP4 plasmid is unable to catabolize octopine and unable to induce crowngall tumours on plants.This publication has 16 references indexed in Scilit:
- Substrate induction of conjugative activity of Agrobacterium tumefaciens Ti plasmidsNature, 1978
- Ti plasmids of Agrobacterium as conjugative plasmidsNature, 1977
- Transfer of virulence in vivo and in vitro in AgrobacteriumNature, 1977
- Acquisition of tumour-inducing ability by non-oncogenic agrobacteria as a result of plasmid transferNature, 1975
- Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing abilityNature, 1974
- The chromosomal integration of a β-lactamase gene derived from the P-type R-factor RP1 inEscherichia coliGenetics Research, 1972
- A Transmissible Resistance Element from a Strain of Pseudomonas aeruginosa Containing No Detectable Extrachromosomal DNAJournal of General Microbiology, 1972
- Relationship between the N function of bacteriophage λ and host RNA polymeraseJournal of Molecular Biology, 1972
- Tumour-initiating Ability and Nutrition in the Genus AgrobacteriumJournal of General Microbiology, 1969
- Integration-negative mutants of bacteriophage lambdaJournal of Molecular Biology, 1968