Interactions Among Phenolics and Peroxidase Isozymes

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Abstract
Peanut [Arachis hypogaea) cells were grown for 14 days in suspension culture before the medium was collected as filtrate. Phenolic compounds were extracted from the medium by liquid-liquid partition technique. Two phenolic compounds, B and F (probably simple acids), were separated by paper chromatography. Isozyme A5, after association with phenolic F, was converted to an isozyme with the same Rm [movement relative to the front] as A2. Isozyme A5 was converted to an intermediate form which, on attachment with a mixture of phenolics B and F, was converted to isozyme A4. A further linking of phenolics B and F to isozyme A4 converted them to an isozyme having the same Rm as isozyme A1.