Acid hydrolases in blister fluid.

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Abstract
Seven glycoside hydrolases [EC 3.2] were investigated in human suction blister fluid, interstitial fluid and in serum. Six of these were characterized; no differences could be demonstrated between the corresponding enzymes from the various sources. The remaining enzyme (.beta.-glucosidase) was not found. Two enzymes (.beta.-acetylglucosaminidase and .beta.-glucuronidase) may diffuse freely from the epidermis into blister fluid, whereas 4 (.alpha.-glucosidase, .alpha.- and .beta.-galactosidase and .alpha.-mannosidase) are almost entirely retained in the roof of the bulla.

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