Epithelial membrane antigen in the cytodiagnosis of effusions and aspirates: Immunocytochemical and ultrastructural localization in benign and malignant cells
- 1 March 1987
- journal article
- research article
- Published by Wiley in Diagnostic Cytopathology
- Vol. 3 (1) , 41-49
- https://doi.org/10.1002/dc.2840030109
Abstract
Immunoreactivity for epithelial membrane antigen (EMA) was evaluated in exfoliated benign and malignant cells using immunoperoxidase and immunogold techniques. In addition, protein A-colloidal gold was used for ultrastructural localization of EMA. Our results suggest that EMA is useful in distinguishing adenocarcinoma cells (strongly positive) from reactive mesothelial cells (negative or weakly positive), lymphoid cells (negative), and a variety of nonepithelial neoplasms (negative) with which they may be confused. Exfoliated cells from two mesotheliomas were also strongly positive for EMA. Ultrastructurally, EMA was distributed in a dense, even, linear pattern along the cell membrane and microvillous surface processes of adenocarcinoma cells. A similar but sparse distribution pattern was observed in reactive mesothelial cells. These observations reflect the increased sensitivity and higher resolution of the immunogold technique. Diagn Cytopathol 1987;3:41–49.Keywords
This publication has 22 references indexed in Scilit:
- Immuno-ultrastructural localization of B-cell-specific monoclonal antibodies B1 and B2.Journal of Histochemistry & Cytochemistry, 1986
- HUMAN LYMPHOID CELLS EXPRESS EPITHELIAL MEMBRANE ANTIGENThe Lancet, 1984
- Immunocytochemical staining with monoclonal antibodies in cytologically "negative" serous effusions from patients with malignant disease.Journal of Clinical Pathology, 1983
- Immunocytochemical staining of cells in pleural and peritoneal effusions with a panel of monoclonal antibodies.Journal of Clinical Pathology, 1983
- USE OF TWO EPITHELIUM-SPECIFIC MONOCLONAL ANTIBODIES FOR DIAGNOSIS OF MALIGNANCY IN SEROUS EFFUSIONSThe Lancet, 1982
- Use of antisera to epithelial membrane antigen for the cytodiagnosis of malignancy in serous effusions.Journal of Clinical Pathology, 1981
- Detection of Small Numbers of Monoclonal B Lymphocytes in the Blood of Patients with LymphomaNew England Journal of Medicine, 1979
- A new antigen on the epithelial membrane: its immunoperoxidase localisation in normal and neoplastic tissue.Journal of Clinical Pathology, 1979
- Ultrastructural localization of intracellular antigens by the use of protein A-gold complex.Journal of Histochemistry & Cytochemistry, 1978
- PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE A NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPYJournal of Histochemistry & Cytochemistry, 1974